376934
C/EBPbeta, NT (CCAAT/Enhancer-Binding Protein beta, NFIL6)
Size
£581.00
- SKU:
- 376934
- Application:
- WB
- Purity:
- Purified by Protein A and Peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to the N-terminal sequence of human C/EBPbeta. Species sequence homology: rat
- Clone:
- LAP
- Format:
- Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors critical for cellular differentiation, terminal functions and inflammatory response (1). Six members of the family have been characterized (C/EBPalpha, -delta, -gamma, -epsilon and -zeta) and are distributed in a variety of tissues (1). There are two forms of C/EBPb, the 38kD liver activating protein (LAP) and the 20kD liver inhibitory protein (LIP) which may be products of alternative translation. The 38kD LAP protein is a transcriptional activator while LIP may act as an inhibitor of C/EBPb transcriptional activity (2). Phosphorylation of C/EBPb at distinct sites stimulates its transcriptional activity (3-5). Phosphorylation at Ser105 of rat C/EBPb, a unique site only present in the rat sequence, seems essential for rat C/EBPb activation (6). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice