VacA, Recombinant, Helicobacter Pylori, aa1076-1290, His-Tag (Vacuolating Cytotoxin Autotransporter)

Size

£633.00

SKU:: 375802
Molecular Weight:: 27.7
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: Induces vacuolation of eukaryotic cells. Causes ulceration and gastric lesions. Partial recombinant protein corresponding to aa1076-1290 from helicobacter pylori Vacuolating Cytotoxin Autotransporter, fused to 6X His-Tag at N-terminal, expressed in E. coli. Swiss/Uniprot: P55981 Molecular Weight: ~27.7kD Amino Acid Sequence: QANSLNSGANNTNFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKSALLRDLNQSYNYLAYSAATRASYGYDFAFFRNALVLKPSVGVSYNHLGSTNFKSNSNQVALKNGSSSQHLFNASANVEARYYYGDTSYFYMNAGVLQEFANFGSSNAVSLNTFKVNAAHNPLSTHARVMMGGELKLAKEVFLNLGFVYLHNLISNIGHFASNLGMRYSF Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.