Serralysin, Recombinant, Proteus Mirabilis, aa17-491, His-SUMO-Tag (ZapA)

Size

£633.00

SKU:: 375279
Molecular Weight:: 68.4
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: One of the virulence factors produced during swarmer cell differentiation of the bacteria, which seems to be associated with pathogenesis. The protease activity is limited to IgA1, IgA2, as well as IgG degradation. Source: Recombinant protein corresponding to aa17-491 from proteus mirabiis zapA, fused to His-SUMO-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~68.4kD Amino Acid Sequence: DLLDKSGIFYNYSTKVLPSFDYDTAGKHIAREDSTWNGKYVIGQPAEVTYSFPKWEGKFNQFGNKNPYEFNELQKEHARKSLDAWSDIANIKFTEVAVGNVDGMKASDVKTDITFGNIYDPNGTFQAYATLPNTYAYGKDLSGQAWFSDYHYAGNTTPELGNYGRLTIIHEIGHTLGLMHPGDYNAGQNVPGYLKSDYAEDSRQYTVMSYWDEYETGAHFQGAYAGAPLLHDISAMQYLYGANTTTRTGDDVYGFNSNTGIDYYTATDSNDKLIFSVWDSGGNDTFDFSGFYQDQLIDLRAGNFSDVGGLQKNVSIAQNVTIENAIGGFGNDIIHGNDADNTLIGGEGDDIIYGHSGNNTIYGGRGQDTLHGGTGSNTFIYKEIADSLVTAADKIMDFKTGIDKIDLSTLIQDTFSSKILNFVDNFTGNAGEATLSYNEVTNASELAINAYGYNYNPDFKIDIVGFVNYETDFIV Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice