DERP1, Dermatophagoides pteronyssinus, aa99-320, Recombinant, 6xHis-SUMO-Tag (Peptidase 1)

Size

£633.00

SKU:: 373040
Molecular Weight:: 41
Purity:: ≥90% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 20mM Tris-HCl, 0.5M sodium chloride, pH 8.0, 6% trehalose. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.
Extra Details:: Thiol protease with a preference for substrates with a large hydrophobic side chain in the P2 position, or with basic residues. Description: E. coli-expressed recombinant protein corresponding to aa99-320 of Dermatophadoides pteronyssinus DERP1 (UniProt accession #P08176), fused to 6xHis-SUMO-Tag at the N-terminus. Molecular Weight: ~41kD Amino Acid Sequence: TNACSINGNAPAEIDLRQMRTVTPIRMQGGCGSCWAFSGVAATESAYLAYRNQSLDLAEQELVDCASQHGCHGDTIPRGIEYIQHNGVVQESYYRYVAREQSCRRPNAQRFGISNYCQIYPPNVNKIREALAQTHSAIAVIIGIKDLDAFRHYDGRTIIQRDNGYQPNYHAVNIVGYSNAQGVDYWIVRNSWDTNWGDNGYGYFAANIDLMMIEEYPYVVIL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.