Macrophage Colony Stimulating Factor Receptor (Macrophage Colony Stimulating Factor 1 Receptor, cFms, M-CSFR, CSF-1R, CD115, Proto-oncogene Csf1r)

Size

£825.00

SKU:: 370311
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to Ala20-Ser511 of mouse Macrophage Colony Stimulating Factor Receptor expressed in NS0 cells. UniProt Accession: P09581
Clone:: 460615
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: M-CSF R (macrophage colony stimulating factor receptor, c-fms) is a 165kD, 977aa type I membrane glycoprotein. It is a member of the type III subfamily of receptor tyrosine kinases that contain five extracellular immunoglobulin-like domains and a cytosolic split kinase domain. M-CSF receptor is expressed primarily on monocytes, macrophages, dendritic cells, and stem cells, and regulates lineage decisions and maturation of these cells. The mouse M-CSF R extracellular domain shares >99% aa identity with rat and 60-63% aa identity with human, dog, cat and cow M-CSF R. It can be released by TACE/ADAM17 cleavage. Applications: Suitable for use in DIrect ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.25ug/10e6 cells using RAW 264.7 mouse monocyte/macrophage cell line Optimal dilution to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice