PDE3A (Phosphodiesterase 3A, cGMP Inhibited, CGI-PDE, Cyclic GMP-inhibited Phosphodiesterase A, cGMP-inhibited 3',5'-cyclic Phosphodiesterase A)

Size

£577.00

SKU:: 369604
Application:: WB
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: Cyclic nucleotide phosphodiesterases (PDEs) comprise a complex group of enzymes, and at least 5 major PDE families or classes with distinctive properties have been identified. Members of the cGMP-inhibited cAMP PDE (cGI-PDE) family, such as PDE3A, are characterized by high affinity for cAMP and cGMP and competitive inhibition of their cAMP hydrolytic activity by cGMP and certain positive inotropic agents. ORF encodes a predicted protein of 125kD. A single mRNA species hybridized with a 4-kb EcoRI restriction fragment of the cDNA containing the entire ORF. The C-terminal region of the deduced aa sequence contains a putative catalytic domain conserved among mammalian PDE families. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.