369589
Neuropeptide Y Receptor Y5 (NPY5R, NPYR5, NPY-Y5 Receptor)
Size
£626.00
- SKU:
- 369589
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Gerald et al. (1996) cloned a novel Y-type receptor that they termed Y5 from rat hypothalamus and reported that it encodes a 456aa protein with less than 35% overall identity to known Y-type receptors. They identified a human Y5 homolog in the GenBank database having 87% overall identity with rat Y5. The rat and human Y5 protein sequences share 88% identity and show 30 to 33% identity to other NPY receptor subtypes. Northern blot analysis revealed that NPY5R is expressed as a 2.6-kb mRNA in the brain but in no other tissues tested. Hu et al. (1996) also isolated another rat cDNA containing a 123aa insertion near the 5-prime end, which may represent an alternatively spliced form of the gene. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice