369553
Insulin Like Protein 5 (INSL5)
Size
£626.00
- SKU:
- 369553
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Insulin gene superfamily hormones regulate cell growth, metabolism, and tissue-specific functions. Members of this family are characterized by a signal peptide, a B chain, a connecting C chain, and an A chain. By EST database searching for sequences related to relaxin (RLN1), Conklin et al. (1999) identified a cDNA encoding INSL5, also designated ZINS3. The deduced 135aa protein, which shares 59% and 40% sequence identity with mouse Insl5 and human RLN1, respectively, has a dibasic cleavage site between the B and C chains. Northern blot analysis revealed expression of an approximately 1.0kb transcript in uterus, rectum, and colon. By radiation hybrid analysis and the use of surrounding markers, Conklin et al. (1999) mapped the INSL5 gene to chromosome 1p31.1-p22.3. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice