369546
HNF1a (Hepatocyte Nuclear Factor 1 Alpha, TCF1, HNF1, LFB1, LF-B1, MODY3, Transcription Factor 1, Hepatic, Hepatic Nuclear Factor, Albumin Proximal Factor, Liver-specific Transcription Factor LF-B1)
Size
£626.00
- SKU:
- 369546
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- HNF1a interacts with sequences required for optimal promoter function of the genes mentioned. The promoter or enhancer regions for several viral and cellular genes not expressed in the liver did not compete for binding to these sequences. HNF1 is predominantly expressed in liver and kidney. HNF1 binds to the promoters of a variety of genes that are expressed exclusively in the liver, e.g., fibrinogen-alpha and -beta, albumin, alpha-fetoprotein, alpha-1-antitrypsin, liver-type pyruvate kinase, transthyretin, aldolase B, and hepatitis B virus large surface protein. HNF1-alpha, which displayed a restricted tissue distribution and did not bind to DNA but, rather, selectively stabilized HNF1-alpha dimers. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice