Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3)

Size

£626.00

SKU:: 369542
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Heparan sulfate (HS) sulfotransferases, such as HS6ST3, modify HS to generate structures required for interactions between HS and a variety of proteins. These interactions are implicated in proliferation and differentiation, adhesion, migration, inflammation, blood coagulation, and other diverse processes. The deduced 470aa protein has characteristics of a type II transmembrane protein, with a short N-terminal segment followed by a hydrophobic sequence. It also contains 6 N-glycosylation sites. Northern blot analysis of adult mouse tissues detected tissue-specific expression of several Hs6st3 transcripts. Hs6st3 was also secreted into the culture medium. Hs6st3 showed 6-O-sulfotransferase activity against HS, N-sulfated heparosan, heparin, and some HS derivatives. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice