FUCa2 (Fucosidase Alpha L2, Plasma, Alpha-L-fucoside Fucohydrolase 2)

Size

£626.00

SKU:: 369525
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: The FUCA2 locus regulates the level of alpha-fucosidase in plasma (Ng et al., 1976) and fibroblasts (Van Elsen et al., 1983) but not in leukocytes. Wood (1979) suggested that the frequency of low plasma fucosidase (62%) makes it a useful marker in linkage studies. Wood (1977) argued that plasma and leukocyte alpha-L-fucosidases are under the same genetic control and that the electrophoretic polymorphism of the enzyme in leukocytes and the enzyme in fucosidosis is determined by mutations at the same locus. In fucosidosis, deficiency of alpha-L-fucosidase is found in both plasma and leukocytes. Normal individuals with low plasma enzyme but normal leukocyte enzyme were described by Ng et al. (1976); however, plasma enzyme is different electrophoretically from leukocyte enzyme. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice