DNASE1L3 (Deoxyribonuclease I Like Protein 3, DHP2, DNAS1L3, LSD, Deoxyribonuclease Gamma, Liver and spleen DNase, DNase I homolog protein DHP2)

Size

£522.00

SKU:: 369504
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: DNASE1L3 encodes a member of the DNase family. The protein hydrolyzes DNA, is not inhibited by actin, and mediates the breakdown of DNA during apoptosis. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. The predicted 306aa protein was 45% identical to that of DNASE1. Northern blot analysis showed that DNASE1L3 was expressed primarily in spleen and liver cells. The predominant mRNA was 1.3kb, although there were 2 other transcripts. The purified recombinant protein had DNase activity in vitro that, unlike that of DNASE1, was not inhibited by G-actin. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice