369498
Deoxythymidylate Kinase, NT (DTYMK, CDC8, TMPK, TYMK, Thymidylate Kinase, dTMP Kinase)
Size
£626.00
- SKU:
- 369498
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa43-190 from human DTYMK, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- Thymidylate kinase (EC 2.7.4.9; ATP:dTMP phosphotransferase) catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway for DNA synthesis. Knowledge of the essential function of dTMP kinase in eukaryotes comes from the study of a cell cycle mutant, cdc8, in Saccharomyces cerevisiae. The product of the cdc8 gene is involved in the elongation step of S phase in cell cycle progression. Huang et al. (1994) cloned the human thymidylate kinase gene by complementation with the cdc8 mutation. The cDNA sequence revealed an open reading frame that encodes a protein with a molecular weight of 23,806. Structural and functional analyses suggested that the cDNA codes for authentic human dTMP kinase. The mRNA levels and enzyme activities corresponded to cell cycle progression and cell growth stages. Applications: Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 5-20ug/ml Immunocytochemistry: 5-20ug/ml Western Blot: 0.5-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice