Aspartyl tRNA Synthetase (DARS, AspRS, PIG40, Aspartate tRNA Ligase 1, Cytoplasmic, Cell proliferation-inducing gene 40 protein)

Size

£626.00

SKU:: 369462
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: DARS (Gly363~Pro501)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: DARS charges its cognate tRNA with aspartate during protein biosynthesis. The predicted DARS protein has 500aa and a calculated molecular mass of 57,000D. The human DARS protein has 95% aa identity with rat Dars and shares unevenly distributed identity with yeast aspartyl-tRNA synthetase, with 69% identity at the C-terminal half and 46% identity at the N-terminal half. The authors discussed several potential functional domains in the DAR protein, including a region of conserved lysine residues which is also found in bacterial and yeast synthetases and is likely to be the binding site for the 3-prime end of tRNA, a nucleotide triphosphate-binding motif, an ATP-binding motif with strong similarity to that present in E. coli alanyl-tRNA synthetase, and a cyclic AMP-dependent protein kinase phosphorylation site. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice