TNF Alpha (Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2)

Size

£422.00

SKU:: 369425
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to bovine TNF alpha.
Clone:: 17D133
Format:: Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Extra Details:: Tumor necrosis factor alpha (TNFalpha, also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNFalpha is produced by a wide variety of immune and epithelial cell types. Bovine TNFalpha consisits of a 35aa cytoplasmic domain, a 21aa transmembrane segment, and a 178aa extracellular domain (ECD). Within the ECD, bovine TNFalpha shares 64%-83% sequence identity with canine, cotton rat, equine, feline, human, mouse, porcine, rat, and rhesus TNFalpha. The 26kD type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer. Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness. Cleavage of membrane bound TNFalpha by TACE/ADAM17 releases a 55kD soluble trimeric form of TNFalpha. TNFalpha trimers bind the ubiquitous TNF RI and the hematopoietic cell restricted TNF RII, both of which are also expressed as homotrimers. TNFalpha regulates lymphoid tissue development through control of apoptosis. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. TNFalpha is a key cytokine in several inflammatory disorders. It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice