Podocalyxin (GCTM-2 Antigen, Gp200, PC, PODXL, PCLP, PCLP1)

Size

£422.00

SKU:: 369402
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human PODXL.
Clone:: 17D110
Format:: Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Extra Details:: Podocalyxin, a sialoglycoprotein, is thought to be the major constituent of the glycocalyx of podocytes. It is a member of the CD34 family of transmembrane sialomucins. It coats the secondary foot processes of the podocytes. It is negatively charged and thus functions to keep adjacent foot processes separated, thereby keeping the urinary filtration barrier open.This function is further supported by knockout studies in mice which reveal an essential role in podocyte morphogenesis. Podocalyxin is also upregulated in a number of cancers and is frequently associated with poor prognosis. Sialylated, O-glycosylated glycoforms of podocalyxin expressed by colon carcinoma cells possess L-selectin and E-selectin binding activity, and may be pivotal to the metastatic spread of colon carcinoma cells. Applications: Suitable for use in Flow Cytometry, ELISA, Immunohistochemistry, Western BlotI, mmunofluorescence and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Western Blot: 1:500-1:5000 Immunohistochemistry (Paraffin): 1:50-1:500 Immunofluorescence: 1:50-1:200 Immunoprecipitation: 1:200-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice