Prion Protein

Size

£960.00

SKU:: 369261
Purity:: Supernatant
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Clone:: 17c43
Format:: Supplied as a liquid in PBS, 4mg/ml BSA, 0.09% sodium azide.
Extra Details:: Prion protein, also known as PRNP, is a ubiquitous membrane glycoprotein whose abnormal self- replicating, misfolded form is widely believed to cause several central nervous system disorders, collectively known as Transmissible Spongiform Encephalopathies (TSE). Applications: Suitable for use in Western Blot, Immunohistochemistry, ELISA, and Fluorescence Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Application Note: Useful in detection agents of transmissible spongiform encephalopathies (TSEs), including sheep scrapie, bovine spongiform encephalopathy (BSE), chronic wasting disease (CWD). Evaluated by immunohistochemistry (IHC) of brain and lymph node from scrapie-infected sheep, and brain and lymph node from a sheep with no known exposure to scrapie. The antibody was diluted to 3-5ug/ml and incubated for 30 minutes with pretreated tissues. Detection was performed on a Ventana immunostainer using biotinylated goat anti-mouse Ig, Sterptavidin-horseradish peroxidase and AEC as the chromogen/substrate. There was no staining of the tissue from the sheep with no exposure to was intense. Tissues for IHC with this antibody are paraffin-embedded formalin-fixed tissues, often decontaminated by incubation of formalin-fixed tissue in formic acid for 60 minutes before re-equilibration in formalin and routine processing and embedding. Three to 5 micron sections are rehydrated, pretreated first with acid (98% formic acid 5-20 minutes, then rinsed and neutralized with several changes of 0.1M Tris buffer, pH 7.5) then with heat (autoclaved at 121°C for 20 minutes in modified citrate buffer, pH 6.1, preferably DAKO Target Petrieval Buffer). Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice