Prion Protein

Size

£1,004.00

SKU:: 369260
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Clone:: 17c39
Format:: Supplied as a liquid in PBS, 4mg/ml BSA, 0.09% sodium azide.
Extra Details:: Prion protein, also known as PRNP, is a ubiquitous membrane glycoprotein whose abnormal self- replicating, misfolded form is widely believed to cause several central nervous system disorders, collectively known as Transmissible Spongiform Encephalopathies (TSE). Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Application Note: Suitable for detecting agents of TSEs in ruminant species. Evaluated by immunohistochemistry (IHC) of brain and lymph node from a scrapie-infected sheep, and brain and lymph node from a sheep with no known exposure to scrapie. The antibody was diluted to 0.5ug/ml and incubated for 30 minutes with pretreated tissues. Dectection was performed on a Ventana immunostainer using biotinylated goat anti-mouse Ig. Streptavidin-horseradish peroxidase and AEC as the chromogen/substrate. There was no staining of the tissue form the sheep with no exposure to scrapie. Staining of the brain and lymph node of the scrapie-infected sheep was intense. Tissues for IHC with this antibody are paraffin-embedded formalin-fixed tissues, often decontaminated by incubation of formalin-fixed tissue in formic acid for 60 minutes before re-equilibration in formalin and routine processing and embedding. Three to 5 micron sections are rehydrated, Pretreated first with acid (98% formic acid 5-20 minutes, then rinsed and neutralized with several changes of 0.1M Tris buffer, pH 7.5) then with heat (autoclaved 121°C for 20 minutes in 0.1M Tris buffer, pH 7.5 or in modified citrate buffer, pH 6.1, such as DAKO Target Retrieval Buffer). Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice