ACPP, Recombinant, Human, aa33-386, His-Tag (Prostatic acid phosphatase Isoform PAP)

Size

£371.00

SKU:: 369014
Molecular Weight:: 41.8
Purity:: ≥95% (SDS-PAGE)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a liquid in PBS, pH 7.4, 10% glycerol.
Extra Details:: ACPP, also known as prostatic acid phosphatase isoform PAP, is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists. The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer. Source: Recombinant protein corresponding to aa33-386 from human ACPP, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~41.8kD (360aa) 40-57KD (SDS-PAGE under reducing conditions) Endotoxin: ~1EU/1ug (LAL) Specific Activity: >100,000units/mg, and is defined as the amount of enzyme that hydrolyze 1nmoles of p-nitrophenyl phosphate (pNPP)/minute at pH 5.0 at 37°C. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.