RF1 (ERF, ETF1, ERF1, TB3-1, D5S1995, SUP45L1)

Size

£652.00

SKU:: 368907
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein fragment corresponding to aa288-437 of human RF1 expressed in E. coli.
Clone:: 4F9H12
Format:: Supplied as a liquid in PBS, 0.05% sodium azide
Extra Details:: This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:200-1:400 ELISA: 1:10,000 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.