PYCARD (ASC, TMS, TMS1, CARD5, TMS-1)

Size

£652.00

SKU:: 368877
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein fragment corresponding to aa1-120 of human PYCARD expressed in E. coli.
Clone:: 1C3D7
Format:: Supplied as a liquid in PBS, 0.05% sodium azide
Extra Details:: This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:200-1:400 ELISA: 1:10,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:200-1:1000 Immunocytochemistry: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice