368525
AIFM1 (Apoptosis-inducing Factor, Mitochondrion-associated 1, AIF, CMTX4, COWCK, COXPD6, PDCD8, Apoptosis-inducing Factor 1, Mitochondrial, Programmed Cell Death 8 (Apoptosis-inducing Factor), Striatal Apoptosis-inducing Factor)
Size
£642.00
- SKU:
- 368525
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- Synthetic peptide corresponding to C-DDPNVTKTLRFKQ of human AIFM1 (NP_004199.1; NP_665811.1; NP_001124319.1). Species sequence homology: bovine
- Format:
- Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
- Extra Details:
- Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10. Applications: Suitable for use in ELISA, Western Blot. Other applications not tested. Recommended Dilution: Peptide ELISA: 1:128,000 Western Blot: 0.1-0.3ug/ml, ~70kD band observed in lysates of cell line Jurkat (calculated MW of 66.9kD according to NP_004199.1). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice