Pyruvate Kinase (Pyruvate kinase muscle isozyme, Cytosolic thyroid hormone-binding protein, Pyruvate kinase isozymes M1/M2)

Size

£709.00

SKU:: 367807
Purity:: Purified by Ion Exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Pyruvate kinase isolated from rabbit Muscle
Format:: Supplied as a lyophilized powder in PBS, pH 7.2, 0.01% sodium azide. Reconstitute with sterile ddH2O.
Extra Details:: Pyruvate kinase is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:4000-1:20,000 Western Blot: 1:500-1:2000 Immunohistochemistry: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.