367762
Plakophilin 2 (PKP2)
Size
£898.00
- SKU:
- 367762
- Purity:
- Supernatant
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa527-872 of human Plakophilin 2 at C-terminal.
- Clone:
- Pkp2-518
- Format:
- Supplied as a liquid, 0.09% sodium azide.
- Extra Details:
- Plakophilin-2 is a protein that in humans is encoded by the PKP2 gene. This gene encodes a member of the arm-repeat (armadillo) and plakophilin gene families. Plakophilin proteins contain numerous armadillo repeats, localize to cell desmosomes and nuclei, and participate in linking cadherins to intermediate filaments in the cytoskeleton. Positive staining was obtained with several carcinomas derived from simple-type epithelia (e.g. hepatomas, colon carcinomas) and some adenocarcinomas derived from stratified epithelia (esophagus carcinoma). Applications: Suitable for use in Immunohistochemistry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunohistochemistry (frozen): for better accessibility of the desmosome-associated antigen frozen sections might be pre-incubated for 5 min in PBS plus 0.2% Triton X-100 prior to antibody application. Longer incubation could result in a loss of Pkp2-nuclear staining. To prevent wash-out of soluble forms of the antigen, fixation with 2% formaldehyde is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice