p16

Size

£539.00

SKU:: 367717
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human p16.
Clone:: DCS-50
Format:: Supplied as a liquid in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide.
Extra Details:: p16 (CDKN2A, p16Ink4A), is key regulator of the cell cycle and involved in cell cycle control and cellular senescence. It is a specific inhibitor for Cdk4 and Cdk6 and binds to the phosphorylated Cdk-cyclin complex. A disruption of this pathway is commonly observed in cancer. p16 is lost in the majority of tumor cell lines and in most primary tumors. It is not expressed in melanoma. In carcinoma driven by an HPV (human papilloma virus) infection, p16 is often overexpressed. Applications: Suitable for use in Immunofluorescence/Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): 1:20-1:100 (1-5ug/ml; microwave treatment recommended) Western Blot: 1:100-1:400 (0.25-1ug/ml) Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.