367488
G-CSF
Size
£581.00
- SKU:
- 367488
- Purity:
- Purified by Protein A affinity chromatography from cell culture.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human G-CSF, expressed in E. coli.
- Clone:
- 16k127
- Format:
- Supplied as a lyophilized powder in PBS, pH 7.4. No preservative added. Reconstitute with 500ul sterile ddH2O.
- Extra Details:
- G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells, including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.2-0.4ug/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant human G-CSF is 1.5-2ng/lane under non-reducing conditions, and 7.5-10ng/lane under reducing conditions. ELISA (100ul/well): 2-4ug/ml will detect at least 100pg/ml of recombinant human G-CSF when used with G8950-26A as the detection antibody at 0.5-1ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice