Carbonic Anhydrase I (Carbonic anhydrase B, YadF, ECK0125, Car1)

Size

£416.00

SKU:: 367325
Purity:: Purified by Ion Exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Carbonic anhydrase I from human Erythrocytes
Format:: Supplied as a lyophilized powder in PBS, pH 7.4, 0.01% sodium azide.
Extra Details:: The carbonic anhydrases (or carbonate dehydratases) form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons (or vice-versa), a reversible reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion; they are therefore classified as metalloenzymes. Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:2000-1:20,000 Western Blot: 1:500-1:2000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.