IgG2a,k Mouse Isotype Control

Size

£229.00

SKU:: 367174
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Clone:: RPC5.4
Format:: Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide
Extra Details:: 367174 is a monoclonal immunoglobulin useful isotype control (negative control) for a comparative expression analysis including flow cytometry, immunoprecipitation, immunohistochemistry and immunocytochemistry in both human and mouse experimental models. Applications: Suitable for use in a comparative expression analysis including Flow Cytometry, Immunoprecipitation, Immunohistochemistry, and Immunocytochemistry in both human and mouse experimental settings. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Application Information: Each lot of purified RPC5.4 has been routinely validated with four serially diluted concentrations to determine any non-specific binding in human PBMCs by flow cytometric analysis. It is suggested to use the same concentration of purified RPC5.4 isotype control immunoglobulin as that of IgG2a, k primary antibody. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.