CD8 (CD8A, Leu2 T Lymphocyte Antigen, MAL, OKT8 T Cell Antigen, p32, T cell Antigen Leu2, T Cell Surface Glycoprotein CD8 alpha, Suppressor/Cytotoxic T Cell) (FITC)

Size

£351.00

SKU:: 367140
Application:: Flow Cytometry
Purity:: ≥90%
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human peripheral blood T lymphocytes
Clone:: OKT8
Format:: Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: CD8 is a 32-34kD type I transmembrane glycoprotein and a member of the immunoglobulin superfamily. CD8alpha forms either a homodimer (2 alpha chains) or a heterodimer with CD8beta and is expressed on the surface of cytotoxic T cells, NK cells, cortical thymocytes, and dendritic cells. CD8 functions as a co-receptor for T cell receptor and specifically binds to class I MHC for antigen recognition and T cell activation. The OKT8 antibody specifically binds the alpha chain of human CD8. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ul labels 10e6 cells in a 100ul staining volume or 100ul of whole blood Optimal dilutions to be determined by the researcher. Excitation Laser: Blue (488nm) Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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