367130
CD7 (T-cell Antigen CD7, GP40, T-cell Leukemia Antigen, T-cell Surface Antigen Leu-9, TP41) (FITC)
Size
£269.00
- SKU:
- 367130
- Application:
- Flow Cytometry
- Purity:
- Highly Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- T-acute lymphoblastic leukemia cells
- Clone:
- 4H9
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- The CD7 antigen is expressed throughout T lymphocyte differentiation, and is present on 85% to 90% of peripheral blood T cells. In normal individuals, the CD7 antibody reacts with all CD8+ T cells, approximately 90% of CD4+ T cells, and most NK cells. CD7 is weakly reactive with monocytes and does not react with granulocytes or B lymphocytes. It is also expressed on 50% of thymocytes. In leukemias, the CD7 antigen is present on the majority of T lymphoid lineages. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ul is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice