CD7 (T-cell Antigen CD7, GP40, T-cell Leukemia Antigen, T-cell Surface Antigen Leu-9, TP41)

Size

£282.00

SKU:: 367125
Application:: Flow Cytometry
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human Leukocytes
Clone:: 124-1D1
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: CD7 is a human T and NK lymphocyte antigen of 40kD. The CD7 antigen is expressed throughout T lymphocyte differentiation, and is present on 85% to 90% of peripheral blood T lymphocytes. In normal individuals, the CD7 antibody reacts with all CD8+ lymphocytes, approximately 90% of CD4+ lymphocytes, and most NK lymphocytes. CD7 is weakly reactive with monocytes and does not react with granulocytes or B lymphocytes. It is expressed on 50% of thymocytes in suspension. In leukemias, the CD7 antigen is present on the majority of T lymphoid lineages. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.5ug/1x10e6 cells in 100ul of staining volume or 0.1ml whole blood followed by a secondary florescent conjugated anti-mouse antibody Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.