CD55 (DAF, Decay accelerating factor)

Size

£326.00

SKU:: 367105
Application:: Flow Cytometry
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human umbilical vein endothelial cells (HUVEC)
Clone:: F4-29D9
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: 367105 binds with a single chain 70kD glycoprotein and identified as CD55 (also known as decay accelerating factor, DAF). CD55 is widely expressed on cells throughout the body including leukocytes, erythrocytes, epithelium, endothelium, and fibroblasts. It is a Glycosyl phosphatidylinositol anchored (GPI- anchored) protein and inhibits autologous complement cascade activation. CD55 also serves as receptor for CD97, echovirus and Coxsackie B virus. CD55 is a useful marker for the diagnosis of paroxysmal nocturnal hemoglobinuria (PNH). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.5ug/1x10e6 cells in 100ul of staining volume followed by a secondary florescent conjugated anti-mouse antibody Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.