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367094

CD45 (Leukocyte Common Antigen, LCA, Ly-5 T200) (Biotin)

Size

£345.00

SKU:
367094
Application:
Flow Cytometry
Purity:
Highly Purified
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Human T lymphocytes
Clone:
F10-89-4
Format:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
Extra Details:
The CD45 antigen is present on all human leucocytes including lymphocytes, monocytes, granulocytes, eosinophils, and basophils in peripheral blood. The antigen has a role in signal transduction, modifying signals from other surface molecules. 367094 has been reported to react weakly with mature circulating erythrocytes and platelets. The antibody recognizes the human leukocyte common antigen which is found on cells from spleen, lymph nodes, 83% bone marrow cells and granulocytes. The antigen recognized is a glycoprotein with a molecular weight of 190kD-215kD. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.5ug/1x10e6 cells in 100ul of staining volume followed by any fluorochrome conjugated streptavidin Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice