CD33 (Myeloid Cell Surface Antigen CD33 Precursor, Sialic Acid Binding Immunoglobulin Like Lectin 3, Siglec 3, Siglec 3, p67) (PE)

Size

£275.00

SKU:: 367078
Application:: Flow Cytometry
Purity:: Highly Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human AML cells
Clone:: WM53
Format:: Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
Extra Details:: This antibody selectively binds with a 67kD glycoprotein, known as CD33 having sialic acid-binding immunoglobulin-like lectin. It belongs to the ITIM (immuno-receptor tyrosine-based inhibitory motif) containing molecules able of recruiting protein tyrosine phosphatases. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ul is found sufficient for staining 1 million PBMCs in 100ul staining volume or 0.1ml whole blood Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.