CD32 (FCGR2A, FCG2, FCGR2A1, IGFR2, Low Affinity Immunoglobulin gamma Fc Region Receptor II-a, CDw32, Fc-gamma RII-a) (FITC)

Size

£248.00

SKU:: 367072
Application:: Flow Cytometry
Purity:: Highly Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: K-562 human erythromyeloblastoid leukemia cell line
Clone:: IV.3
Format:: Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: 367072 reacts with a low affinity receptor for the Fc of IgG (FcgammaRII, CD32) that is expressed on human platelets, monocytes, granulocytes and B cells. CD32 also cross-reacts with granulocytes and monocytes from peripheral blood lymphocytes from non-human primates. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ul is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.