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367069

CD3 (T3E, T-cell Surface Antigen T3/Leu-4 epsilon Chain) (PerCP)

Size

£273.00

SKU:
367069
Application:
Flow Cytometry
Purity:
Highly Purified
Storage Conditions:
4°C Do Not Freeze
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Human peripheral blood lymphocytes
Clone:
OKT3
Format:
Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with PerCP.
Extra Details:
CD3epsilon is a 20kD chain, which together with CD3lambda, CD3delta, and CD3zeta, and a T cell receptor (alpha/beta or gamma/sigma) form the CD3/T-cell receptor complex. It is a specific marker for T lymphocytes, NK T cells, and some thymocytes. Crosslinking of TCR initiates an intracellular signaling cascade resulting in cellular activation and proliferation. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proved effective in the treatment of renal, heart, and liver allograft rejection. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ul is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. PerCP conjugates are sensitive to light.
Shipping Conditions:
Blue Ice