367062
CD25 (IL2RA, Interleukin-2 Receptor Subunit alpha, TAC Antigen, p55) (FITC)
Size
£244.00
- SKU:
- 367062
- Application:
- Flow Cytometry
- Purity:
- Highly Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Influenza virus stimulated human peripheral blood mononuclear cells
- Clone:
- 7G7B6
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- The antibody specifically binds to the 55kD type I transmembrane glycoprotein known as the low-affinity interleukin-2 receptor alpha chain subunit (IL2Ra, CD25). CD25 is expressed on regulatory T cells and on activated lymphocytes (T and B) and monocytes. CD25 expression on T and B lymphocytes is upregulated by antigenic or mitogenic stimulation. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ul is sufficient for staining of 1 million cells in a 100ul staining volume or 100ul of whole blood Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice