CD19 (B Lymphocyte Antigen CD19, Differentiation Antigen CD19, B4, CVID3) (Biotin)

Size

£288.00

SKU:: 367040
Application:: Flow Cytometry
Purity:: Highly Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human CCL (chronic lymphocytic leukemia) cells
Clone:: 4G7
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: The CD19 antigen is present on approximately 7% to 23% of human peripheral blood lymphocytes and on spleenocytes, and on human B lymphocytes at all stages of maturation but is lost on terminally differentiated plasma cells. CD19 does not react with resting or activated T lymphocytes, granulocytes, or monocytes. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.5ug/1x10e6 cells in 100ul of staining volume followed by any fluorochrome conjugated streptavidin Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.