367035
CD16 (Fc gamma RIII, NK cells) (FITC)
Size
£332.00
- SKU:
- 367035
- Application:
- Flow Cytometry
- Purity:
- Highly Purified
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human peripheral blood T lymphocytes
- Clone:
- 3G8
- Format:
- Supplied as a liquid in PBS, pH 7.2, 0.2% BSA, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- CD16 binds to the 50-65kD transmembrane protein of IgG FcgammaRIII, an surface antigen commonly associated with human NK cells. Besides surface expression on NK cells, it is also expressed on activated monocytes, macrophages, on neutrophils and placental trophoblasts in humans. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC). CD16 is also useful in experiments related to immunohistochemical staining, immunoprecipitation, NK cell proliferation, blocking of phagocytosis and blocking of immunoglobulin binding through FcgammaRIII. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.5ug/1x10e6 cells in 100ul of staining volume followed by a secondary florescent conjugated anti-mouse antibody Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice