CD14 (Monocyte Differentiation Antigen CD14, Myeloid Cell-Specific Leucine-Rich Glycoprotein)

Size

£288.00

SKU:: 367027
Application:: Flow Cytometry
Purity:: Highly Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Cultured human peripheral blood monocytes
Clone:: 26ic
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: CD14 is a 53-55kD glycosylphosphatidylinositol (GPI)-anchored and single chain glycoprotein expressed at high levels on monocytes. Additionally, CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells and some Langerhans cells. The binding of CD14 antibody does not inhibit CD14 mediated activities, and is useful for detecting CD14 expression by immunofluorescence and or immunocytochemical methods. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.5ug/1x10e6 cells in 100ul of staining volume followed by any fluorescent conjugated anti-mouse secondary antibody Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.