ZBTB46, NT (ZNF340, Zinc Finger and BTB Domain-containing Protein 46, BTB-ZF Protein Expressed in effector lymphocytes, BTB/POZ Domain-containing Protein 4, BZEL, Zinc Finger Protein 340)

Size

£761.00

SKU:: 367006
Purity:: Purified by Protein G affinity chromatography
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant internal protein fragment corresponding to human ZBTB46 at N-terminal, fused to GST-Tag
Clone:: 5A8.1
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide
Extra Details:: Zinc finger and BTB domain-containing protein 46 (UniProt Q86UZ6; also known as BTB-ZF protein expressed in effector lymphocytes, BTB/POZ domain-containing protein 4, BZEL, Classical dendritic cell-restricted zinc finger transcription factor zDC, Zinc finger protein 340) is encoded by the ZBTB46 (also known as BTBD4, ZNF340) gene (Gene ID 140685) in human. ZBTB46/ZNF340 is a broad complex, tramtrack, bric-a-brac and zinc finger (BTB-ZF) protein expressed in classical dendritic cells (cDCs), but not other immune cell populations, including plasmacytoid DCs, monocytes, or macrophages. Genome-wide ChIP-seq analysis reveals that ZBTB46/ZNF340 associates with more than 1,000 gene promoters and acts as a transcriptional repressor against many of these target genes. Zbtb46-knockout profoundly alters the cDC subset composition in the spleen in favor of CD8+ DCs, as well as upregulates activation pathways in steady-state cDCs, including elevated creb1 and MHC II expression, and enhances cDC production of vascular endothelial growth factor, leading to increased vascularization of skin-draining lymph nodes. Human ZBTB46/ZNF340 is a 589-a.a. protein containing a BTB domain (aa31-99) and two C2H2-type Zinc fingers (aa418-436, 446-468). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml detects ZBTB46 in 10ug of human ovary tissue lysate at ~70kD. Target band size appears larger than the calculated molecular weight of 64.08kD. Uncharacterized bands may be observed in some lysates. Immunohistochemistry (Paraffin): 1:50-1:250 detects ZBTB46 in human cererbral cortex, kidney, and tonsil tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice