367000
TOE1, CT (Target of EGR1 Protein 1)
Size
£800.00
- SKU:
- 367000
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Linear peptide corresponding to human TOE1 at C-terminal.
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Extra Details:
- Target of EGR1 protein 1 (UniProt Q96GM8) is encoded by the TOE1 gene (Gene ID 114034) in human. Originally characterized and thereby named as a downstream target of the immediate early gene Egr1, TOE1 is a nuclear protein localized primarily in nucleoli and Cajal bodies. TOE1 overexpression causes an upregulation of the cyclin-dependent kinase inhibitor p21 and cell cycle arrest at G2/M. TOE1 also interacts wtih p53 C-terminal tetramerization domain and enhances p53 transcriptional activity. In addtion, TOE1 exhibits inhibitory activity against HIV-1 replication and evidence indicates TOE1 interaction with the viral specific transactivator response element as part of the inhibitory mechanism. TOE1 is upregulated and secreted by activated CD8(+) T lymphocytes, and TOE1 can be proeolytically cleaved by granzyme B at D328 and D363, generating a 35aa (aa329-363) fragment containing the nuclear localization signal/NLS (aa335-347). The 35aa fragment retains both the antiviral potency and cell-penetrating capability. Human TOE1 is produced as a 510aa protein, containing a C3H1-type Zinc finger (aa294-322) and anuclear localization signal/NLS (a.a.335-347). The initiation methionine is removed posttranslationally. Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Immunoprecipitation: 1ul immunoprecipitated TOE1 from 20ug of HEK293T cell lysate Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice