366997
Tau, oligomeric (T22) (Microtubule-associated Protein tau Oligomer, Tau Oligomer, PHF-tau Oligomer, Paired Helical Filament-tau Oligomer, Neurofibrillary Tangle Protein Oligomer)
Size
£953.00
- SKU:
- 366997
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human Tau-441 (Tau-F, Tau-4, 2N4R isoform) oligomers.
- Format:
- Supplied as a liquid in PBS. No preservative added.
- Extra Details:
- Microtubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU, MAPT1, MTBTL) gene (Gene ID 4137) in human. In Alzheimer's disease (AD) pathology, accumulation of the microtubule-associated protein tau takes place primarily in the neurons. Tau accumulates in both the somatodendritic and axonal domains of neurons. Tau also accumulates in the soma as neurofibrillary tangles (NFTs). Cell death and synaptic lesions occur independently of NFT formation, and research indicates that NFT formation alone is insufficient for neurodegeneration, suggesting that soluble tau aggregates may be the more toxic and pathologically significant tau species. Tau oligomers are neurotoxic when applied extracellularly to cultured neuronal cells, and tau oligomers (but not fibrils) induce neurodegeneration and synaptic and mitochondrial dysfunction in vivo. Moreove, researchers are able to use tau oligomers as a reliable biomarker to differentiate AD brains from age-matched non-AD brains. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Dot Blot. Other applications not tested. Recommended Dilutions: Dot Blot: 2ug/ml detects 100ng of in vitro generated recombinant human tau oligomers, but not tau monomer. Western Blot: 4-6.67ug/mL detects tau oligomers in frontal cortex tissue lysates from Alzheimer's diseased (AD), but not from age-matched non-AD, human brains Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice