PSAT1 (Phosphoserine Aminotransferase, Phosphohydroxythreonine Aminotransferase, PSAT)

Size

£800.00

SKU:: 366985
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Linear peptide corresponding to a sequence within the region between the two pyridoxal phosphate-binding domains of human PSAT1, KLH conjugated. Species sequence homology: rabbit
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Phosphoserine aminotransferase (EC 2.6.1.52; UniProt Q9Y617; also known as Phosphohydroxythreonine aminotransferase, PSAT) is encoded by the PSAT1 (also known as NLS2, PSA, PSATD) gene (Gene ID 29968) in human. PSAT1 is a class-V pyridoxal-phosphate-dependent aminotransferase family member that catalyzes the reversible conversion of 3-phosphohydroxypyruvate and to 1-phosphoserine, as well as the conversion of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to 4-phosphonooxy-L-threonine, using, in both cases, L-glutamate as an amine donor, which is converted to 2-oxoglutarate. PSAT1 is involved in serine biosynthesis from glycolytic intermediates and PSAT1 upregulation is reported in non-small cell lung cancer (NSCLC) and colon cancer cells, while PSAT1 knockdoown is shown to suppress the proliferation of MDA-MB-468 and BT-20 breast cancer cells. Applications: Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunocytochemistry: 1:250, detects PSAT1 in A431, HeLa, HepG2, and NIH/3T3 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice