Platelet Factor 4 (Platelet Factor 4, C-X-C Motif Chemokine 4, Iroplact, Oncostatin-A, PF-4)

Size

£800.00

SKU:: 366983
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human platelet PF4
Clone:: 197.2
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Platelet factor 4 (UniProt P02776; also known as C-X-C motif chemokine 4, Iroplact, Oncostatin-A, PF-4) is encoded by the PF4 (also known as CXCL4, SCYB4) gene (Gene ID 5196) in human. Platelet factor 4 (PF4) is a small CXC chemokine family cytokine released from alpha-granules of activated platelets during platelet aggregation to promote blood coagulation. PF4 binds heparin with high affinity (Kd ~4-20 nM) via its heparin-binding domain (aa92-98) and neutralizes the anti-thrombin activity of heparin-like molecules. PF4 is chemotactic for neutrophils, fibroblasts and monocytes, and interacts with the chemokine receptor CXCR3 splice variant CXCR3B. In individuals suffering from heparin-induced thrombocytopenia (HIT), PF4-heparin complexes can trigger an immune response and the generation of anti-PF4 antibodies, resulting in the foramtion of macromolecular antibody-heparin-PF4 immune complexes that bind Fc RIIa (CD32a) on platelets and induce platelet activation. Platelet activation in turn results in more PF4 release into circulation, enhanced immune-complex formation, exaggerated platelet activation, and cell clearance. PF4 is synthesized with a signal peptide sequence (aa1-31) that is removed posttranslationally to produce the mature PF4 (aa32-101), additional proteolytic cleavage yields the PF4 short form (aa48-101). Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug detected PF4 in one million 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized human platelets Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice