O-GlcNAc (beta-GlcNAc, beta-linked N-acetylglucosamine, O-GlcNAc, O-linked beta-GlcNAc)

Size

£800.00

SKU:: 366976
Purity:: Purified by Ion Exchange chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Nuclear pore complex-lamina fraction isolated from rat liver nuclear envelopes.
Clone:: RL1
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Posttranslational modification of proteins by -linked N-acetylglucosamine ( -GlcNAc) via the hydroxyl moieties on serine or threonine residues is termed O-linked -GlcNAc or simply O-GlcNAc. O-GlcNAc is one of the most abundant posttranslational modifications within the nucleocytoplasmic compartments of all animals and plants. Unlike other types of protein glycosylations, O-GlcNAc occurs exclusively within the nuclear and cytoplasmic compartments and is generally not further modified to form more elongated structures. In addition, O-GlcNAcylation is a highly dynamic and reversible process. The O-GlcNAc transferase (OGT) attaches O-GlcNAc to proteins at specific serine or threonine residues, while O-GlcNAcase catalyzes the removal/hydrolysis of O-GlcNAc from proteins. In fact, a dynamic interplay between O-GlcNAcylation and serine/threonine phosphorylation plays an important role in regulating cellular signaling. Tau and RNA polymerase II (Pol II) are two well known proteins that undergo modification by O-GlcNAcylation. In Alzheimer s diseased human brains, tau becomes extensively phosphorylated and less O-GlcNAcylated. Similarly, O-GlcNAc is removed and replaced with O-phosphate on the Poly II CTD when the elongation phase of transcription is initiated. Applications: Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Affinity Binding Assay, Electron Microscopy, Inhibition. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:50 detects O-GlcNAcylated cytoplasmic and nuclear pore proteins in rat kidney and spleen tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice