NKCC1/2, CT (Solute Carrier Family 12 Member 1, Solute Carrier Family 12 Member 2, Bumetanide-sensitive Sodium-(potassium)-chloride Cotransporter 2, Bumetanide-sensitive Sodium-(potassium)-chloride Cotransporter 1, Basolateral Na-K-Cl Symporter)

Size

£800.00

SKU:: 366975
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein fragment corresponding to human NKCC1 at C-terminal.
Clone:: T4
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Solute carrier family 12 member 1 (UniProt Q13621; a.k.a. Bumetanide-sensitive sodium- (potassium)-chloride cotransporter 2) and solute carrier family 12 member 2 (UniProt P55011; a.k.a. Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1) are encoded by the SLC12A1 (a.k.a. NKCC2; Gene ID 6557) and SLC12A2 (a.k.a. NKCC1; Gene ID 6558) gene, respectively, in human. NKCC1 (SLC12A2) and NKCC2 (SLC12A1) belong to the subfamily of cation-chloride cotransporters (CCCs) that mediate electroneutral transport of sodium, potassium and chloride across the plasma membrane and are inhibited by bumetanide and furosemide-type of compounds. NKCCs play an important role in cell volume regulation, where NKCC2 is involved in reabsorption of salt and osmotically-obliged water in renal epithelial cells, while NKCC1 has a major impact on intracellular chloride ion concentration ([Cl-]i) in vascular smooth muscle cells (VSMCs) and neuronal cells. In primary hypertension, NKCC1 activity is increased in VSMCs and paraventricular nucleus (PVN) neurons, leading to elevated peripheral resistance in systemic circulation and activation of sympathetic nerve system (SNS), respectively. Human NKCC1 and NKCC2 are 12-transmembrane proteins having their N- and C-terminal ends exposed at the cytoplasmic side (aa1-285 and 921-1212 of NKCC1; aa1-177 and 814-1099 of NKCC2). Applications: Suitable for use in Electron Microscopy, Immunofluorescence, Immunohistochemistry, Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:3000 detects NKCC in human prostate cancer, as well as normal colon, small intestine, and testis tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice