366961
IMPACT, CT (Protein IMPACT, Imprinted and Ancient Gene Protein Homolog)
Size
£761.00
- SKU:
- 366961
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- KLH-conjugated, linear peptide corresponding to the C-terminal sequence of human IMPACT.
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Extra Details:
- Protein IMPACT (UniProt Q9P2X3; also known as Imprinted and ancient gene protein homolog) is encoded by the IMPACT gene (Gene ID 55364) in human. IMPACT is a cytoplasmic protein preferentially expressed in neurons. IMPACT is highly abundant in the hypothalamus and in scattered neurons in other areas, such as the hippocampal interneurons. IMPACT and its yeast ortholog Yih1 contain an RWD domain (aa14-116 of human and mouse IMPACT) that interacts with GCN1 and competes against GCN2 for GCN1 binding. IMPACT and Yih1 overexpression in mouse embryonic fibroblasts (MEFs) and yeasts inhibits GCN2 activation induction. IMPACT is a developmentally regulated protein that promotes neuritogenesis, while GCN2 strongly inhibits spontaneous neurite outgrowth. Increased abundance of IMPACT in differentiated neuronal cells enhances translation initiation by preventing GCN2 signaling. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:250, detects IMPACT in human prostate cancer, as well as in normal human cerebral cortex and mouse cerebellum tissue sections. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice