HSD17B3 (Testosterone 17-beta-dehydrogenase 3, 17-beta-HSD 3, 17-beta-hydroxysteroid Dehydrogenase Type 3, Short Chain Dehydrogenase/reductase Family 12C Member 2, Testicular 17-beta-hydroxysteroid Dehydrogenase)

Size

£825.00

SKU:: 366957
Application:: WB
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human HSD17B3. Species sequence homology: human (100%)
Clone:: 3E11.2
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Testosterone 17-beta-dehydrogenase 3 (EC 1.1.1.64; UniProt P37058; also known as 17-beta-HSD 3, 17-beta-hydroxysteroid dehydrogenase type 3, Short chain dehydrogenase/reductase family 12C member 2, Testicular 17-beta-hydroxysteroid dehydrogenase) is encoded by the HSD17B3 (also known as EDH17B3, SDR12C2) gene (Gene ID 3293) in human. 17 -hydroxysteroid dehydrogenases (17 -HSDs) mediate the activation and deactivation of sex steroids involving redox reactions centered about the C17 area of the steroid backbone. 17 -HSD3 catalyzes the final step in the steroidogenesis of the potent androgen testosterone (T) by selectively reducing the C17 ketone of 4-dione, utilizing NADPH as a cofactor, in testis and prostate. Upregulated 17 -HSD3 mRNA level is found in cancerous prostate biopsies when compared to normal tissue, while 17 -HSD3 deficiency caused by HSD17B3 gene mutations results in 46,XY Disorders of Sex Development (46,XY DSD). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000 detects HSD17B3 in 10ug of mouse testis tissue lysate Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice