CD278 (Inducible T-Cell Costimulator, ICOS, Activation-inducible Lymphocyte Immunomediatory Molecule, CCLP, CD278, CD28 and CTLA-4-like Protein, CD28-related Protein 1, CRP-1, H4)

Size

£800.00

SKU:: 366934
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Hamster
Immunogen:: D10.G4.1 mouse TH2 cells. Epitope: Extracellular domain. Species sequence homology: rhesus macque.
Clone:: C398.4A
Format:: Supplied as a liquid in PBS. No preservative added.
Extra Details:: Inducible T-cell costimulator (UniProt Q9WVS0; also known as Activation-inducible lymphocyte immunomediatory molecule, CCLP, CD278, CD28 and CTLA-4-like protein, CD28-related protein 1, CRP-1, H4) is encoded by the Icos (also known as Ailim) gene (Gene ID 54167) in murine species. ICOS is a costimulatory molecule expressed on activated T cells and regulatory T cells, it plays a crucial role in T-cell survival and function, Th2 cell differentiation, and lung inflammatory responses. Upon ICOS-ligand (ICOS-L) binding, ICOS initiates a cascade of intracellular signaling events, leading to the production of cytokines such as IL-4 and IL-13. Both ICOS and ICOS-L are expressed on type 2 innate lymphoid cells (ILC2s) that, along with Th2 cells, contribute to the pathogenesis of asthma by producing copious amounts of IL-5 and IL-13. ICOS and ICOS-L interaction is reported to promote cytokine production and survival of ILC2s through STAT5 signaling. ICOS-deficiency or blocking ICOS and ICOS-L interaction is shown to prevent cytokine-induced asthma and lung inflammation in a humanized murine model with adoptively transferred human peripheral ILC2s. Human and murine ICOS proteins are produced with a signal peptide (aa1-20) sequence, which is removed posttranslationally to yield the mature protein with a large N-terminal extracellular (human aa21-140 and murine aa21-144) region composed almost entirely of a V-type Ig-like domain (human aa30-132 and murine aa30-133), followed by a transmembrane domain (human aa141-161 and murine aa145-165) and a cytoplasmic tail (human aa162-199 and murine aa166-200). Applications: Suitable for use in Flow Cytometry, Function Assay, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.1ug detects ICOS/CD278 immunoreactivity on the surface of PHA-activated (5ug/mL at 37°C for 72 hours) human PBMCs. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice